Bio Engineer Archive

Thread: Need Help with hacking Bases.....

Impslayer2004
Tue May 17, 2005 10:55 am
#1
I have searched and read the few posts here..... I am more confused now than before.


Can anyone provide a simple and easy to understand guide? Please dont point me to the one with all the pictures.... My brain melted after trying to figure that out.


Any help would be much appreciated.
Dorelli
Wed May 18, 2005 12:20 am
#2








Impslayer2004 wrote:

I have searched and read the few posts here..... I am more confused now than before.


Can anyone provide a simple and easy to understand guide? Please dont point me to the one with all the pictures.... My brain melted after trying to figure that out.


Any help would be much appreciated.




Well ... how about this ... (sorry this is from memory but it is a quick explanation and mostly correct):


1. you obviously have to wait your turn (otherwise you can't get at the terminal) - sorry to state the obvious but ... hey ...why not be thorough.


2. Radial dial the terminal that is yours. I cannot remember what it is called. You will get the option to download a DNA sequence (I think).


3. What you will see is a list of A,G,C,T's in a 'random' order and at the top another list ... the top list you cannot select, the bottom 'list' you can. It is important to think of the botton list as being a LIST - read it from the top to the bottom. Think of the top sequence as something you have in your hand and you are trying to lie it down on top of the other one (call it the sample sequence). The long list that is selectable does not change (call it the master sequence). The one 'in your hand' does change every time you select a match.


4. You MUST match the first letter (it is called a 'base') of the sequence you have 'in your hand' (ie. at the top of the box).


  • If it is an A click on a T.

  • If it is a T click on an A.

  • If it is a G click on a C.

  • If it is a C click on a G.

(this correlates to how RL DNA matches up with base-pairs but it doesn't matter). A's want to match T's and vice versa, G's want to match C's and vice versa.


5. Now if you imagine you taking the sequence 'in your hand' and lying it on top of the other you might get something like this:


A T C G T T A
! !! !
A C C A C T T GC A G G T T A A C G


so your sample sequence matches up with 4 of the letters on the 'master sequence' - and you have clicked on about the 6th letter down the list in the master sequence. BUT! You could have clicked on ANY 'T' in the master sequence - it doesn't matter ... some will give you more matches than others depending on how the 2 sequences line up is all.


6. Now you will get a new 'sample sequence' to match. The ones you have already matched on the master sequence will be taken out of action b/c they have got their match but they are just ignored. You can still 'lay your sample' over them and it will match the unpaired letters and ignore the paired ones.


(NOTE: in RL this would not happen - the other sequence would be either there or not there and if there it would block but nm).


7. Keep doing this until you have matched all the pairs in the master sequence. It will get harder as you go on but keep doing it letter by letter and you will get your matches. If nothing matches the first letter then just go quickly onto the next sample sequence.


Good luck and geez - i hope you ain't an imp! Oh duh - i just read your tag again - nm...


Dorelli

Message Edited by Dorelli on 05-17-2005 12:46 PM



Dorelli Deacon of Bloodfin server
RIP BioEngineering 15-11-2005 : RIP CH 15-11-2005

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